Journal: International Urogynecology Journal

Article Title: Local immune response in bladder pain syndrome/interstitial cystitis ESSIC type 3C

doi: 10.1007/s00192-013-2112-0

Figure Lengend Snippet: Statistics for gene expression (RT-qPCR)

Article Snippet: The following TaqMan® Gene Expression Assays (4331182) were analysed: cytotoxic T-lymphocyte-associated protein 4 ( CTLA4 , Hs03044418_m1) as a T-cell marker; CD20 (membrane-spanning 4-domains, subfamily A, member 1 MS4A1 , Hs00544819_m1) and CD79A molecule, immunoglobulin-associated alpha ( CD79A , Hs00233566_m1) as B-cell markers; immunoglobulin heavy locus ( IGH@ , Hs00378230_g1) as a marker for antibody heavy chain gene expression; and uroplakin 1B ( UPK1B , Hs00199583_m1), uroplakin 3A ( UPK3A , Hs00199590_m1) and cytokeratin 20 ( KRT20 , Hs00300643_m1) as urothelial markers.

Techniques: Gene Expression

Journal: International Urogynecology Journal

Article Title: Local immune response in bladder pain syndrome/interstitial cystitis ESSIC type 3C

doi: 10.1007/s00192-013-2112-0

Figure Lengend Snippet: Cut-off values and accuracy of biomarkers for BPS/IC ESSIC type 3C

Article Snippet: The following TaqMan® Gene Expression Assays (4331182) were analysed: cytotoxic T-lymphocyte-associated protein 4 ( CTLA4 , Hs03044418_m1) as a T-cell marker; CD20 (membrane-spanning 4-domains, subfamily A, member 1 MS4A1 , Hs00544819_m1) and CD79A molecule, immunoglobulin-associated alpha ( CD79A , Hs00233566_m1) as B-cell markers; immunoglobulin heavy locus ( IGH@ , Hs00378230_g1) as a marker for antibody heavy chain gene expression; and uroplakin 1B ( UPK1B , Hs00199583_m1), uroplakin 3A ( UPK3A , Hs00199590_m1) and cytokeratin 20 ( KRT20 , Hs00300643_m1) as urothelial markers.

Techniques:

Journal: Pharmaceutics

Article Title: Bacterial Cellulose as Drug Delivery System for Optimizing Release of Immune Checkpoint Blocking Antibodies

doi: 10.3390/pharmaceutics14071351

Figure Lengend Snippet: Cytotoxicity evaluation of BC extracts in MC38 tumor cells with 7-AAD staining and MTS. BC extracts were prepared by dissolving 1 g of BC in 50 mL culture medium, which yielded an extraction ratio of 20 mg/mL. Empty extracts or extracts supplied with human IgG or anti-CTLA-4 (both starting at a concentration of 50 µg/mL) were tested on MC38 cells. DMSO was used as positive cell killing control. The primary measure was cell viability, which was assessed by 7-AAD staining (live-dead cells exclusion marker; figures a – c ). Higher 7-AAD MFI signal is a hallmark of dying cells with leaky cell membranes. Cytotoxicity was also assessed using a cell metabolism assay (MTS), in which higher cell metabolism is indicative of higher cell viability ( d – f ). Cell metabolism was measured 48 h after incubation. All data are shown as mean ± SD for triplicates. Only the DMSO treatment significantly decreased cell viability, which was assessed with a Student’s t test, with **** denoting p < 0.0001.

Article Snippet: BC fleeces were loaded with human IgG antibody (50 µg) or anti-CTLA-4 (Syrian hamster IgG, clone 9H10; Bioxcell, Lebanon, NH, USA; 100 µg) according to .

Techniques: Staining, Concentration Assay, Marker, Incubation

Journal: Pharmaceutics

Article Title: Bacterial Cellulose as Drug Delivery System for Optimizing Release of Immune Checkpoint Blocking Antibodies

doi: 10.3390/pharmaceutics14071351

Figure Lengend Snippet: BC reduces serum antibody levels in vivo. Photographs in ( a ) depict in chronological order the procedures of the BC implantation, starting with loading, placing the BC sample underneath the skin, wound closure, visual inspection of the skin and removing the BC implant at D21 (after the mice were killed). Body weight measurements are depicted in ( b ), for the BC-treated mice, the body weight differences at various time-points were compared with the initial body weight at D0. Body weight differences were assessed with a paired Student’s t -test, with NS denoting no statistical differences compared to the body weights at D0. In ( c ) and ( d ), the serum IgG and anti-CTLA-4 levels are shown for several time-points after treatment, respectively. Statistical differences between the BC and PBS group were assessed with an unpaired Student’s t -test and are denoted as * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: BC fleeces were loaded with human IgG antibody (50 µg) or anti-CTLA-4 (Syrian hamster IgG, clone 9H10; Bioxcell, Lebanon, NH, USA; 100 µg) according to .

Techniques: In Vivo

Journal: Cell Reports Medicine

Article Title: Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma

doi: 10.1016/j.xcrm.2021.100399

Figure Lengend Snippet:

Article Snippet: InVivoPlus anti-mouse CTLA-4 (CD152) antibody, clone 9D9 , BioXCell , Cat# BE0164, RRID: AB_10949609.

Techniques: Recombinant, Infection, Transfection, Cell Isolation, Selection, Staining, Sequencing, Transgenic Assay, Expressing, Software

Journal: Immunity

Article Title: PD-L1:CD80 Cis -Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways

doi: 10.1016/j.immuni.2019.11.003

Figure Lengend Snippet: (A) A Jurkat-Raji co-culture assay analyzing how PD-L1 interferes with CTLA-4-mediated CD80 depletion. Cartoons on the left depict the co-cultured cells. On the immediate right are representative flow-cytometry histograms of CD80 expression (anti-CD80 allophycocyanin) on Raji cells before (0 h) and after co-culture (0.5 h). Further on the right are representative confocal images for the Jurkat-Raji conjugate (scale bars, 10 μm). Rightmost is a bar graph showing CD80 MFI of Raji at 0.5 h, normalized to CD80 MFI at 0 h (mean ± SEM, n = 4).

Article Snippet: This home-made SNAP–CTLA-4–His 6 and human CD80–His, purchased from Sino Biological, were subjected to gel filtration chromatography, and monomeric fractions of each collected and used in the SPR assays.

Techniques: Co-culture Assay, Cell Culture, Flow Cytometry, Expressing, Co-Culture Assay

Journal: Immunity

Article Title: PD-L1:CD80 Cis -Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways

doi: 10.1016/j.immuni.2019.11.003

Figure Lengend Snippet: (A) Representative flow-cytometry histograms of CTLA-4-huFc staining of the indicated types of Raji cells. Bound CTLA-4-huFc was labeled by AF647 anti-human IgG Fc, the MFI of which was plotted against (CTLA-4-huFc). Shown in gray are Raji (CD80+CD86−) cells stained by isolated huFc domain. Means ± SEM, n ≥ 3.

Article Snippet: This home-made SNAP–CTLA-4–His 6 and human CD80–His, purchased from Sino Biological, were subjected to gel filtration chromatography, and monomeric fractions of each collected and used in the SPR assays.

Techniques: Flow Cytometry, Staining, Labeling, Isolation